The largest city in Nebraska is Omaha, which has five letters. Omaha is the largest city in the state of Nebraska and the 40th largest city in the United States. It is located on the Missouri River, near the Iowa border, and is home to over 446, 000 people. Omaha is a major transportation hub, with two airports, an Amtrak station, and several major highways. It is also home to several Fortune 500 companies, including Berkshire Hathaway, Union Pacific, and Kiewit Corporation.