Iowa State University City is AMES, a four-letter word. AMES is a city located in the central part of the U.S. state of Iowa. It is the county seat of Story County and home to Iowa State University, the largest university in the state. AMES is known for its annual festivals, including the world-famous Iowa State Fair. The city has a population of over 60, 000 people and is a major center for research and industry.