The two French cities on the Rhone River are Lyon and Avignon. Lyon is the third largest city in France and is located in the east-central part of the country. It is known for its historical and architectural landmarks, such as the Basilica of Notre-Dame de Fourvière and the Roman amphitheater. Avignon is located in the south of France and is known for its medieval architecture, including the Palais des Papes and the Pont d'Avignon. Both cities are popular tourist destinations and offer a variety of cultural activities.